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Gene therapy is performed by ____.


A) replacing the genomes of an individual with a brand-new set of genomes with the goal of treating a genetic defect
B) replacing a defective gene in an individual with a normal gene with the goal of treating a genetic disorder
C) transferring a mutated gene into an individual with the goal of treating a genetic defect
D) transferring a normal or a modified gene into an individual with the goal of treating a genetic defect
E) replacing a defective enzyme in an individual with a normal enzyme with the goal of treating a genetic disorder

F) C) and D)
G) B) and C)

Correct Answer

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Golden Rice is made more β -carotene rich with genes from bacteria and ____.


A) corn
B) mice
C) tulips
D) daffodils
E) oranges

F) A) and B)
G) C) and D)

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A

Which most accurately describes the expression "recombinant DNA"?


A) DNA molecule cloned from a given organism
B) DNA molecule that contains genetic information from more than one organism
C) DNA molecule that is moved from one tube to another in a laboratory
D) DNA molecule isolated from a specific organism
E) DNA molecule that has been used in a laboratory for experimental purposes

F) A) and D)
G) C) and E)

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A DNA molecule that can accept foreign DNA and be replicated inside a host cell is known as a ____.


A) genetic probe
B) DNA library
C) recombinant DNA
D) cloning vector
E) restriction enzyme

F) B) and E)
G) B) and D)

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Most genetic engineering involves the use of ____.


A) yeast and plants
B) plants and E. coli
C) yeast and mice
D) bacteria and mice
E) yeast and bacteria

F) All of the above
G) C) and D)

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In agriculture, the use of GMOs is regulated by the ____.


A) FDA
B) AHA
C) EPA
D) USDA
E) NIH

F) A) and E)
G) A) and C)

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The E4 variation of the APOE gene increases a person's chances of developing ____.


A) cystic fibrosis
B) Parkinson's disease
C) Huntington's disease
D) Alzheimer's disease
E) Duchenne's muscular dystrophy

F) C) and D)
G) A) and E)

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Which procedure is used to amplify DNA in the laboratory?


A) DNA cloning
B) reverse transcription
C) nucleic acid hybridization
D) DNA restriction
E) polymerase chain reaction (PCR)

F) A) and C)
G) B) and D)

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β -Carotene is converted to vitamin ____ in the cells of the small intestine.


A) B12
B) A
C) E
D) C
E) D

F) B) and C)
G) C) and D)

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How much Golden Rice will supply a child's daily need for the vitamin that is converted from β -carotene?


A) two teaspoons
B) a half of a cup
C) one cup
D) one quart
E) one pint

F) A) and D)
G) A) and C)

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C

Crops have been genetically modified to resist the herbicide ____.


A) glyphosate
B) DDT
C) triazine
D) 2,4-D
E) atrazine

F) C) and E)
G) None of the above

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Which is a complete list of the ingredients that are essential for PCR?


A) nucleotides, DNA template, Taq polymerase, and primers
B) nucleotides, DNA template, DNA ligase, and primers
C) restriction enzymes, DNA template, Taq polymerase, and primers
D) nucleotides, DNA template, Taq polymerase, and plasmids
E) nucleotides, DNA template, DNA ligase, and plasmids

F) B) and D)
G) A) and D)

Correct Answer

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The technique of ____ analyzes 500,000 SNPs in an individual's genome.


A) electrophoresis
B) cutting and pasting
C) DNA cloning
D) PCR
E) SNP-chips

F) B) and C)
G) B) and E)

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If the nucleotide sequence ACTGAG represents the sticky end of a DNA molecule, to what other sticky end sequence would it base pair?


A) TGACTC
B) UGACUC
C) ACTGAG
D) ACUGUG
E) GAGTCA

F) None of the above
G) B) and E)

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A

The increased reliance on genetically engineered crops grew from the need to ____.


A) develop new species of crop plants
B) provide farmers with more expensive seeds
C) find new pesticides
D) produce more food at lower cost without harming the environment
E) find more ways to profit from crop plants

F) A) and B)
G) C) and E)

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During a DNA profiling analysis, short tandem repeats are first ____.


A) subjected to high heat
B) inserted into a plasmid
C) chopped up using a restriction enzyme
D) placed on a gel for electrophoresis
E) amplified using PCR

F) A) and B)
G) All of the above

Correct Answer

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Sticky ends are so named because ____.


A) they are single-stranded and will base pair with complementary sequences
B) covalent bonds spontaneously form between them to form a continuous DNA molecule
C) primers hybridize to them during the first step of PCR
D) they hybridize to a DNA sequence of interest
E) they stick to invading viral DNA

F) A) and E)
G) B) and E)

Correct Answer

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After DNA unwinds and becomes single-stranded in a PCR reaction, the temperature is lowered to allow the ____ to hybridize with the DNA template.


A) vectors
B) primers
C) tracers
D) restriction enzymes
E) DNA ligase

F) D) and E)
G) C) and D)

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When a gene from one species is transferred into another species, the result is ____.


A) a GMO
B) a new species
C) a hybrid animal
D) eugenics
E) a lethal mutation

F) D) and E)
G) A) and B)

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What is a transgenic organism?


A) an organism with multiple copies of the same gene
B) an organism that has genes from another species
C) an organism that does not use DNA to specify proteins
D) an organism that has had its entire genome sequenced
E) an organism that has been DNA profiled

F) A) and D)
G) B) and C)

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